Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury

Rick Kapur; Michael Kim; Johan Rebetz; Björn Hallström; Jonas T. Björkman; Alisa Takabe-French; Noel Kim; Jonathan Liu; Shanjeevan Shanmugabhavananthan; Stefan Milosevic; Mark J. McVey; Edwin R. Speck; John W. Semple

Blood Advances (Jul 2018)

Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury

Rick Kapur,
Michael Kim,
Johan Rebetz,
Björn Hallström,
Jonas T. Björkman,
Alisa Takabe-French,
Noel Kim,
Jonathan Liu,
Shanjeevan Shanmugabhavananthan,
Stefan Milosevic,
Mark J. McVey,
Edwin R. Speck,
John W. Semple

Affiliations

Rick Kapur: Division of Hematology and Transfusion Medicine, Lund University, Lund, Sweden;
Michael Kim: Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada;; The Toronto Platelet Immunobiology Group, St. Michael's Hospital, Toronto, ON, Canada;
Johan Rebetz: Division of Hematology and Transfusion Medicine, Lund University, Lund, Sweden;
Björn Hallström: Center for Translational Genomics, Lund University, Lund, Sweden;
Jonas T. Björkman: Center for Molecular Diagnostics, Skåne University Hospital, Lund, Sweden;
Alisa Takabe-French: Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada;; The Toronto Platelet Immunobiology Group, St. Michael's Hospital, Toronto, ON, Canada;
Noel Kim: Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada;; The Toronto Platelet Immunobiology Group, St. Michael's Hospital, Toronto, ON, Canada;
Jonathan Liu: Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada;; The Toronto Platelet Immunobiology Group, St. Michael's Hospital, Toronto, ON, Canada;
Shanjeevan Shanmugabhavananthan: Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada;; The Toronto Platelet Immunobiology Group, St. Michael's Hospital, Toronto, ON, Canada;
Stefan Milosevic: Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada;; The Toronto Platelet Immunobiology Group, St. Michael's Hospital, Toronto, ON, Canada;
Mark J. McVey: Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada;; The Toronto Platelet Immunobiology Group, St. Michael's Hospital, Toronto, ON, Canada;; Canadian Blood Services, Toronto, ON, Canada;; Department of Anesthesia, University of Toronto, Toronto, ON, Canada;; Department of Physiology, University of Toronto, Toronto, ON, Canada;; Department of Anesthesia and Pain Medicine, Hospital for Sick Children, Toronto, ON, Canada;
Edwin R. Speck: Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada;; The Toronto Platelet Immunobiology Group, St. Michael's Hospital, Toronto, ON, Canada;
John W. Semple: Division of Hematology and Transfusion Medicine, Lund University, Lund, Sweden;; Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, ON, Canada;; The Toronto Platelet Immunobiology Group, St. Michael's Hospital, Toronto, ON, Canada;; Canadian Blood Services, Toronto, ON, Canada;; Department of Pharmacology, University of Toronto, Toronto, ON, Canada; Department of Medicine, University of Toronto, Toronto, ON, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada; John W. Semple, Lund University, BMC C14, Klinikgatan 26, 221 84 Lund, Sweden;

Journal volume & issue: Vol. 2, no. 13
pp. 1651 – 1663

Abstract

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Abstract: Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress upon blood transfusion and is the leading cause of transfusion-related fatalities. Whether the gut microbiota plays any role in the development of TRALI is currently unknown. We observed that untreated barrier-free (BF) mice suffered from severe antibody-mediated acute lung injury, whereas the more sterile housed specific pathogen-free (SPF) mice and gut flora-depleted BF mice were both protected from lung injury. The prevention of TRALI in the SPF mice and gut flora–depleted BF mice was associated with decreased plasma macrophage inflammatory protein-2 levels as well as decreased pulmonary neutrophil accumulation. DNA sequencing of amplicons of the 16S ribosomal RNA gene revealed a varying gastrointestinal bacterial composition between BF and SPF mice. BF fecal matter transferred into SPF mice significantly restored TRALI susceptibility in SPF mice. These data reveal a link between the gut flora composition and the development of antibody-mediated TRALI in mice. Assessment of gut microbial composition may help in TRALI risk assessment before transfusion.

Published in Blood Advances

ISSN: 2473-9529 (Print); 2473-9537 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine
Website: https://ashpublications.org/bloodadvances

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