Foxo in T Cells Regulates Thermogenic Program through Ccr4/Ccl22 Axis
Tetsuhiro Kikuchi,
Jun Nakae,
Yoshinaga Kawano,
Nobuyuki Watanabe,
Masafumi Onodera,
Hiroshi Itoh
Affiliations
Tetsuhiro Kikuchi
Navigation Medicine of Kidney and Metabolism, Division of Endocrinology, Metabolism, and Nephrology, Department of Internal Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan
Jun Nakae
Navigation Medicine of Kidney and Metabolism, Division of Endocrinology, Metabolism, and Nephrology, Department of Internal Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan; Department of Physiology, International University of Health and Welfare School of Medicine, Narita 286-8686, Japan; Corresponding author
Yoshinaga Kawano
Navigation Medicine of Kidney and Metabolism, Division of Endocrinology, Metabolism, and Nephrology, Department of Internal Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan
Nobuyuki Watanabe
Department of Human Genetics, National Center for Child Health and Development, Tokyo 157-8535, Japan
Masafumi Onodera
Department of Human Genetics, National Center for Child Health and Development, Tokyo 157-8535, Japan
Hiroshi Itoh
Navigation Medicine of Kidney and Metabolism, Division of Endocrinology, Metabolism, and Nephrology, Department of Internal Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan
Summary: Crosstalk between immunity and the thermogenic program has provided insight into metabolic energy regulation. Here, we generated thermogenic program-accelerating mice (T-QKO), in which Foxo1 is knockout and Foxo3 is hetero-knockout in CD4+ T cells. T-QKO exhibit lean phenotype under HFD due to increased energy expenditure. Cold exposure significantly increased expression of the thermogenic genes (Ppargc1a and Ucp1), Th2 cytokines (Il4 and Il13), and Th2 marker gene (Gata3) in subcutaneous adipose tissue (SC) of T-QKO. Furthermore, Ccr4 expression was significantly increased in Th2 cells of T-QKO, and cold exposure induced Ccl22 expression in SC, leading to increased accumulation of Th2 cell population in SC of T-QKO. These data reveal a mechanism by which cold exposure induces selective recruitment of Th2 cells into SC, leading to regulation of energy expenditure by generating beige adipocyte and suggest that inhibition of Foxo in T cells may support a strategy to prevent and treat obesity. : Molecular Mechanism of Behavior; Immunology; Immune Response; Diabetology Subject Areas: Molecular Mechanism of Behavior, Immunology, Immune Response, Diabetology
