Investigation of membrane protein—protein interactions using correlative FRET-PLA

Abstract

Fluorescence resonance energy transfer (FRET) analysis and the recently developed proximity ligation assay (PLA) are widely used to study protein—protein interactions in situ. We have developed correlative FRET-PLA to monitor interactions between membrane proteins that frequently cause problems in confirmatory co-immunoprecipitation assays. Correlative FRET-PLA is particularly aimed at delivering robust and reliable results and is useful for investigating protein—protein interactions.

Keywords

• FRET
• PLA
• protein-protein interaction
• HIV-1
• CD63