EXPRESSION OF CANCER-ASSOCIATED PROTEINS IN TISSUES AND BLOOD SERUM FROM PATIENTS WITH RENAL CELL CARCINOMA

Abstract

Abstract. Samples of renal cell carcinoma (RCC) and adjacent normal tissues were studied by means of immunoblotting, using monoclonal antibodies [(MKA 1, 1F3-2D4, IgG1 class), MKA 2H6, IgG1 class] against membrane proteins of HEp-2 cells (larynx cancer). Strong reactivity of MKA 1 was revealed with p34-38 (13/34, 38%) and p28-29 (7/34, 21%), and, generally, for 20 of 34 tumor samples(59%, p<0,05). The differences in antigen expression in renal cell carcinomas are statistically significant, as compared with adjacent (presumably normal) tissues. The p34-38 expression was not revealed in serum pellets obtained from 4 cancer patients by ultracentrifugation. A variety of multiple proteins was revealed in one оf seventeen tissue samples surrounding the tumor (normal breast tissue). Interestingly, a difference was found between renal cell carcinoma (p28-29 expression) and adjacent normal tissues (p21-24) that was revealed by means of MKA 2H6. The p21-24 and p28-29 may represent a product of p34-38 posttranslational modifications. It was supposed that a variety of proteins revealed with MKA 1, 2H6 are interrelated, due to identity of some sites. Detection of p34-38, p28-29 with MKA 1 may be potentially used as a supplementary immunological approach, in order to differentiate renal cell carcinoma tissues of epithelial origin from the adjacent tissues. Further studies are needed to elucidate an opportunity of MKA 2H6 usage, aiming for detection of p28-29 in renal cell carcinoma samples, like as p21-24 for specimens of adjacent normal tissues. (Med. Immunol., 2008, vol. 10, N 2-3, pp 209-214).

Keywords

• renal cell carcinomas
• monoclonal antibodies
• immunoblotting