Zunyimycins B and C, New Chloroanthrabenzoxocinones Antibiotics against Methicillin-Resistant Staphylococcus aureus and Enterococci from Streptomyces sp. FJS31-2
Yuhong Lü,
Meiyun Shao,
Yinyin Wang,
Shengyan Qian,
Miao Wang,
Yingquan Wang,
Xiaoqian Li,
Yuxin Bao,
Chengmin Deng,
Changwu Yue,
Daishun Liu,
Ning Liu,
Minghao Liu,
Ying Huang,
Zehui Chen,
Yonglin Hu
Affiliations
Yuhong Lü
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China
Meiyun Shao
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China
Yinyin Wang
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China
Shengyan Qian
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China
Miao Wang
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China
Yingquan Wang
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China
Xiaoqian Li
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China
Yuxin Bao
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi 563003, Guizhou, China
Chengmin Deng
Zunyi Key Laboratory of Genetic Diagnosis & Targeted Drug Therapy, The First People’s Hospital of Zunyi, Zunyi 563003, Guizhou, China
Changwu Yue
Zunyi Key Laboratory of Genetic Diagnosis & Targeted Drug Therapy, The First People’s Hospital of Zunyi, Zunyi 563003, Guizhou, China
Daishun Liu
Zunyi Key Laboratory of Genetic Diagnosis & Targeted Drug Therapy, The First People’s Hospital of Zunyi, Zunyi 563003, Guizhou, China
Ning Liu
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Minghao Liu
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Ying Huang
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Zehui Chen
Department of Clinical Laboratory Medicine, Zunyi Medical University, Zunyi 563003, Guizhou, China
Yonglin Hu
Department of Clinical Laboratory Medicine, Zunyi Medical University, Zunyi 563003, Guizhou, China
This study performed an optimization of the fermentation conditions to activate the expression of the zunyimycin family biosynthesis genes of the zunyimycin-producing streptomycetes strain Streptomyces sp. FJS31-2. Bioassay-guided isolation and purification by varied chromatographic methods yielded two new compounds of the zunyimycin derivatives, namely, 31-2-7 and 31-2-8, accompanied with three known anthrabenzoxocinones family members of zunyimycin A, BE24566B, and chloroanthrabenzoxocinone. Their structures were elucidated by NMR, HRESIMS, IR, UV, and CD. Results showed that these two compounds were structurally similar to the previously reported compound zunyimycin A but differed in positions and number of chlorine atom substitution. The two novel compounds were called zunyimycins B and C. Antibacterial activity assay indicated that zunyimycin C showed a good inhibitory effect on the methicillin-resistant Staphylococcus aureus and Enterococci.
