PLoS ONE (Jan 2017)

Deep learning approach to bacterial colony classification.

  • Bartosz Zieliński,
  • Anna Plichta,
  • Krzysztof Misztal,
  • Przemysław Spurek,
  • Monika Brzychczy-Włoch,
  • Dorota Ochońska

DOI
https://doi.org/10.1371/journal.pone.0184554
Journal volume & issue
Vol. 12, no. 9
p. e0184554

Abstract

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In microbiology it is diagnostically useful to recognize various genera and species of bacteria. It can be achieved using computer-aided methods, which make the recognition processes more automatic and thus significantly reduce the time necessary for the classification. Moreover, in case of diagnostic uncertainty (the misleading similarity in shape or structure of bacterial cells), such methods can minimize the risk of incorrect recognition. In this article, we apply the state of the art method for texture analysis to classify genera and species of bacteria. This method uses deep Convolutional Neural Networks to obtain image descriptors, which are then encoded and classified with Support Vector Machine or Random Forest. To evaluate this approach and to make it comparable with other approaches, we provide a new dataset of images. DIBaS dataset (Digital Image of Bacterial Species) contains 660 images with 33 different genera and species of bacteria.