Characterization of Autochthonous Strains from the Cecal Content of Creole Roosters for a Potential Use as Probiotics
Elvia Guadalupe Melara,
Mavir Carolina Avellaneda,
Ana Julia Rondón,
Marlen Rodríguez,
Manuel Valdivié,
Yordan Martínez
Affiliations
Elvia Guadalupe Melara
Sustainable Tropical Agriculture, Zamorano University, San Antonio de Oriente, Francisco Morazán, Tegucigalpa 11101, Honduras
Mavir Carolina Avellaneda
Plant Pathology, Diagnosis and Molecular Research Laboratory, Agricultural Sciences and Production Department, Zamorano University, San Antonio de Oriente, Francisco Morazán, Tegucigalpa 11101, Honduras
Ana Julia Rondón
Centro de Estudios Biotecnológicos, Facultad de Ciencias Agropecuarias, Universidad de Matanzas, Autopista Varadero km 3 ½, Matanzas 44740, Cuba
Marlen Rodríguez
Centro de Estudios Biotecnológicos, Facultad de Ciencias Agropecuarias, Universidad de Matanzas, Autopista Varadero km 3 ½, Matanzas 44740, Cuba
Manuel Valdivié
National Center for Laboratory Animal Production, Santiago de las Vegas, Rancho Boyeros, La Habana 10100, Cuba
Yordan Martínez
Poultry Research and Teaching Center, Agricultural Science and Production Department, Zamorano University, San Antonio de Oriente, Francisco Morazán, Tegucigalpa 11101, Honduras
Five strains (CLP2, CLP3, CLP4, CLP5, and CLP6) were isolated from the cecal content of Creole roosters fed without antibiotic growth promoters. Biochemical and morphological tests (negative catalase and oxidase) confirmed the presence of lactic acid bacteria. Additionally, considering the 16s RNA, Lactobacillus vaginalis (CLP2, CLP3, CLP5, and CLP6) and Lactobacillus reuteri (CLP4) were identified. All strains (mainly CLP4 and CLP5) showed variable and significant growth (p Escherichia coli ATCC® 11775TM, Salmonella Typhimurium ATCC® 14028TM, and Clostridium perfringens ATCC® 13124TM. In addition, CLP4 had lower sensitivity in the presence of amoxicillin and tetracycline compared to these pathogenic bacteria. Considering these in vitro results, it is necessary to carry out in vivo studies with the CLP4 strain to test the hypothesis of its probiotic effect in poultry.
