Nature Communications (Jan 2021)

High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replication

  • Jessica S. Williams,
  • Percy P. Tumbale,
  • Mercedes E. Arana,
  • Julian A. Rana,
  • R. Scott Williams,
  • Thomas A. Kunkel

DOI
https://doi.org/10.1038/s41467-020-20800-1
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 11

Abstract

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DNA ligase 1 (LIG1) finalizes eukaryotic nuclear DNA synthesis by sealing Okazaki fragments using DNA end-joining reactions. Here the authors, by studying an engineered low-fidelity LIG1, reveal that LIG1 is a highly accurate DNA ligase in vivo.