The article deals with the problem of the influence of distance learning on the level of physical fitness of students. The use of modern information and communication technologies has led to the creation of a new educational environment, which, as part of the training, has provided students with the opportunity to independently acquire knowledge in the context of constantly updated information. The discipline «Physical Education» is one of the main practical classes in the university program, and in order to assess how the new proposed training conditions will affect the quality of physical fitness of students, we will conduct a study within the framework of physical education classes. The aim of the study is to analyze the impact of distance learning on the level of physical fitness of students. Objectives of the study: to consider the content of distance learning, to analyze the passing of the standard running 100 m., to conduct an experiment and check the impact of distance learning on the level of physical fitness of students, to draw conclusions and make recommendations. The research methods are the generalization of scientific and methodological literature, the method of mathematical statistics, and the pedagogical experiment. Organization and results of the study. The study was conducted with students of the 1st year of full-time study at the Perm State Technical University. As a result of the experiment, it was revealed that during distance learning, the theoretical part of the students mastered better, and in the practical part (running 100 m.), the results deteriorated, since most of the physical culture program was presented in the form of theoretical and methodological-practical sections, and there was no possibility of active and systematic running, mastering technical and tactical training techniques and improving them.