Nature Communications (Feb 2018)

Mapping molecular assemblies with fluorescence microscopy and object-based spatial statistics

  • Thibault Lagache,
  • Alexandre Grassart,
  • Stéphane Dallongeville,
  • Orestis Faklaris,
  • Nathalie Sauvonnet,
  • Alexandre Dufour,
  • Lydia Danglot,
  • Jean-Christophe Olivo-Marin

DOI
https://doi.org/10.1038/s41467-018-03053-x
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 15

Abstract

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Elucidating molecular organisation requires precise localisation and analysis. Here the authors develop SODA software for automatic and quantitative mapping of statistically coupled molecules, and use it to unravel spatial organisation of thousands of synaptic proteins in SIM and 3DSTORM microscopy.