Sorbitol MacConkey agar and wastewaters` coliphages

Tesfaye Bedada; Teshome Eshete; Adey Desta; Fitsum Yifat; Dagim Birri; Kassu Tullu; Samson Gebre; Firehiwot Dera; Kaleab Awoke; Tatek Feto; Waktole Sima; Elias Deribe; Dejenie Teklu; Getinet Irena; Geremew Weledesemayat; Tigist Negassi; Asnake Garedew

doi:10.22126/arww.2024.9527.1305

Journal of Applied Research in Water and Wastewater (Aug 2023)

Sorbitol MacConkey agar and wastewaters` coliphages

Tesfaye Bedada,
Teshome Eshete,
Adey Desta,
Fitsum Yifat,
Dagim Birri,
Kassu Tullu,
Samson Gebre,
Firehiwot Dera,
Kaleab Awoke,
Tatek Feto,
Waktole Sima,
Elias Deribe,
Dejenie Teklu,
Getinet Irena,
Geremew Weledesemayat,
Tigist Negassi,
Asnake Garedew

Affiliations

Tesfaye Bedada: Public Health Microbiology Research Team, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Teshome Eshete: Gondar University, Gondar, School of Biomedical and Laboratory Sciences, Ethiopia.
Adey Desta: Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Ethiopia.
Fitsum Yifat: Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Ethiopia.
Dagim Birri: Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Ethiopia.
Kassu Tullu: Department of Medical Laboratory Science, College of Health Sciences, Addis Ababa. University, Ethiopia.
Samson Gebre: Public Health Microbiology Research Team, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Firehiwot Dera: Public Health Microbiology Research Team, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Kaleab Awoke: Public Health Microbiology Research Team, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Tatek Feto: Public Health Microbiology Research Team, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Waktole Sima: Public Health Microbiology Research Team, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Elias Deribe: Clinical Bacteriology and Mycology Reference Laboratory, Ethiopian Public Health Institute, Ethiopia.
Dejenie Teklu: Clinical Bacteriology and Mycology Reference Laboratory, Ethiopian Public Health Institute, Ethiopia.
Getinet Irena: Public Health Microbiology Research Team, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Geremew Weledesemayat: Public Health Microbiology Research Team, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Tigist Negassi: Public Health Microbiology Research Team, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Asnake Garedew: Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Ethiopia.

DOI: https://doi.org/10.22126/arww.2024.9527.1305
Journal volume & issue: Vol. 10, no. 2
pp. 112 – 118

Abstract

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A total of 114 samples collected from hospital wastewaters and rivers in Addis Ababa, Ethiopia were tested for non-sorbitol fermenting bacteria and coliphages. Sorbitol MacConkey agar is mainly used in the detection of E. coli O157:H7. However, other emerging diarrhoeagenic enteropathogens such as Plesiomonas shigelloides, Edwardsiella tarda, Providencia alcalifaciens, Escherichia albertii, Escherichia vulneris and Escherichia fergusonii were detected in the samples using this medium. Information for most of the emerging enteropathogens is scarce in most countries including Ethiopia. A total of 20 different genera, 38 species of non-sorbitol fermenting bacteria were isolated. Escherichia coli O157 could not be detected from any of the samples. All these backgrounds may mask the detection of Escherichia coli O157. Even if sorbitol MacConkey agar has several background limitations, different emerging diarrhoeagenic non-sorbitol fermenting bacteria were detected in the majority of the rivers and hospitals` wastewaters samples. The correlation between coliphages and non-sorbitol fermenting bacteria were not significant. As several bacteria have been isolated on sorbitol MacConkey agar medium, it is essential that the most selective laboratory techniques will be desired for outbreak investigation of E. coli O157, but other non-sorbitol fermenting enteropathogens should also be detected using sorbitol MacConkey agar in low resources countries.

Published in Journal of Applied Research in Water and Wastewater

ISSN: 2476-6283 (Online)
Publisher: Razi University
Country of publisher: Iran, Islamic Republic of
LCC subjects: Technology: Environmental technology. Sanitary engineering
Website: http://arww.razi.ac.ir/

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