Comparative Analysis of the Transcriptional Response of Tolerant and Sensitive Wheat Genotypes to Drought Stress in Field Conditions

Abstract

Drought stress is one of the most adverse environmental limiting factors for wheat (Triticum aestivum L.) productivity worldwide. For better understanding of the molecular mechanism of wheat in response to drought, a comparative transcriptome approach was applied to investigate the gene expression change of two wheat cultivars, Jimai No. 47 (drought-tolerant) and Yanzhan No. 4110 (drought-sensitive) in the field under irrigated and drought-stressed conditions. A total of 3754 and 2325 differential expressed genes (DEGs) were found in Jimai No. 47 and Yanzhan No. 4110, respectively, of which 377 genes were overlapped, which could be considered to be the potential drought-responsive genes. GO (Gene Ontology) analysis showed that these DEGs of tolerant genotype were significantly enriched in signaling transduction and MAP (mitogen-activated protein) kinase activity, while that of sensitive genotype was involved in photosynthesis, membrane protein complex, and guard cell differentiation. Furthermore, 32 and 2 RNA editing sites were identified in drought-tolerant and sensitive genotypes under drought compared to irrigation, demonstrating that RNA editing also plays an important role in response to drought in wheat. This study investigated the gene expression pattern and RNA editing sites of two wheat cultivars with contrasting tolerance in field condition, which will contribute to a better understanding of the molecular mechanism of drought tolerance in wheat and beyond.

Keywords

• differentially expressed genes
• drought
• RNA-seq
• RNA editing
• wheat