Establishment of a transgene-free iPS cell line (SDCHi007-A) from a young patient bearing a ATP1A2 mutation and suffering from Epilepsy

Hongwei Zhang; Tong Zhang; Yaping Wang; Jianguo Shi; Yao Meng; Qi Zhang; Qingwei Guo; Chunhong Duan

Stem Cell Research (Sep 2024)

Establishment of a transgene-free iPS cell line (SDCHi007-A) from a young patient bearing a ATP1A2 mutation and suffering from Epilepsy

Hongwei Zhang,
Tong Zhang,
Yaping Wang,
Jianguo Shi,
Yao Meng,
Qi Zhang,
Qingwei Guo,
Chunhong Duan

Affiliations

Hongwei Zhang: Epilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, China
Tong Zhang: Epilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, China
Yaping Wang: Epilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, China
Jianguo Shi: Epilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, China
Yao Meng: Epilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, China
Qi Zhang: Epilepsy Center, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Epilepsy Center, Jinan Children’s Hospital, Jinan, Shandong 250022, China
Qingwei Guo: Hematology and Oncology Department, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Hematology and Oncology Department, Jinan Children’s Hospital, Jinan, Shandong 250022, China; Corresponding authors at: Hematology and Oncology Department, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China (Q. Guo). Respiratory Department, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China (C. Duan).
Chunhong Duan: Respiratory Department, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China; Respiratory Department, Jinan Children’s Hospital, Jinan, Shandong 250022, China; Corresponding authors at: Hematology and Oncology Department, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China (Q. Guo). Respiratory Department, Children’s Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China (C. Duan).

Journal volume & issue: Vol. 79
p. 103490

Abstract

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Epilepsy is a chronic neurological disease. Here we describe the generation of induced pluripotent stem cells (iPSCs) from a patient diagnosed as epilepsy caused by ATP1A2 gene mutation. Induced pluripotent stem cells (iPSCs) were developed using non-integrating episomal vectors containing OCT4, SOX2, KLF4, BCL-XL and C-MYC. The established iPSC line (SDCHi007-A) displayed pluripotent cell morphology, high expression levels of pluripotency markers, differentiation potential in vitro, normal karyotype, and remaining the original ATP1A2 gene mutation.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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