Synthesis and Characterization of Calcium Silicate Nanoparticles Stabilized with Amino Acids
Anastasiya A. Blinova,
Abdurasul A. Karamirzoev,
Asiyat R. Guseynova,
David G. Maglakelidze,
Tatiana A. Ilyaeva,
Batradz A. Gusov,
Avetis P. Meliksetyants,
Mari M. Pirumian,
Maxim A. Taravanov,
Maxim A. Pirogov,
Dmitriy S. Vakalov,
Tatiana V. Bernyukevich,
Alexey A. Gvozdenko,
Andrey A. Nagdalian,
Andrey V. Blinov
Affiliations
Anastasiya A. Blinova
Department of Physics and Technology of Nanostructures and Materials, Physical and Technical Faculty, North Caucasus Federal University, 355017 Stavropol, Russia
Abdurasul A. Karamirzoev
Faculty of Dentistry, North Ossetian State Medical University, 362025 Vladikavkaz, Russia
Asiyat R. Guseynova
Faculty of Dentistry, Derzhavin Tambov State University, 392008 Tambov, Russia
David G. Maglakelidze
Department of Physics and Technology of Nanostructures and Materials, Physical and Technical Faculty, North Caucasus Federal University, 355017 Stavropol, Russia
Tatiana A. Ilyaeva
Faculty of Medicine, Stavropol State Medical University, 355017 Stavropol, Russia
Batradz A. Gusov
Faculty of Dentistry, North Ossetian State Medical University, 362025 Vladikavkaz, Russia
Avetis P. Meliksetyants
Faculty of Dentistry, North Ossetian State Medical University, 362025 Vladikavkaz, Russia
Mari M. Pirumian
Medical and Preventive Faculty, Rostov State Medical University, 344022 Rostov-on-Don, Russia
Maxim A. Taravanov
Department of Physics and Technology of Nanostructures and Materials, Physical and Technical Faculty, North Caucasus Federal University, 355017 Stavropol, Russia
Maxim A. Pirogov
Department of Physics and Technology of Nanostructures and Materials, Physical and Technical Faculty, North Caucasus Federal University, 355017 Stavropol, Russia
Dmitriy S. Vakalov
Department of Physics and Technology of Nanostructures and Materials, Physical and Technical Faculty, North Caucasus Federal University, 355017 Stavropol, Russia
Tatiana V. Bernyukevich
Moscow State University of Civil Engineering, 129337 Moscow, Russia
Alexey A. Gvozdenko
Department of Physics and Technology of Nanostructures and Materials, Physical and Technical Faculty, North Caucasus Federal University, 355017 Stavropol, Russia
Andrey A. Nagdalian
Laboratory of Food and Industrial Biotechnology, North Caucasus Federal University, 355017 Stavropol, Russia
Andrey V. Blinov
Department of Physics and Technology of Nanostructures and Materials, Physical and Technical Faculty, North Caucasus Federal University, 355017 Stavropol, Russia
This work presents the development of a method for the synthesis of calcium silicate nanoparticles stabilized with essential amino acids. CaSiO3 nanoparticles were obtained through chemical precipitation. In the first stage, the optimal calcium-containing precursor was determined. The samples were examined using scanning electron microscopy. It was found that Ca(CH3COO)2 was the optimal calcium-containing precursor. Then, the phase composition of calcium silicate was studied using X-ray phase analysis. The results showed the presence of high-intensity bands in the diffractogram, which characterized the phase of the nanosized CaSiO3—wollastonite. In the next stage, the influence of the type of amino acid on the microstructure of calcium silicate was studied. The amnio acids studied were valine, L-leucine, L-isoleucine, L-methionine, L-threonine, L-lysine, L-phenylalanine, and L-tryptophan. The analysis of the SEM micrographs showed that the addition of amino acids did not significantly affect the morphology of the CaSiO3 samples. The surface of the CaSiO3 samples, both without a stabilizer and with amino acids, was represented by irregularly shaped aggregates consisting of nanoparticles with a diameter of 50–400 nm. Further, in order to determine the optimal amino acid to use to stabilize nanoparticles, computerized quantum chemical modeling was carried out. Analysis of the data obtained showed that the most energetically favorable interaction was the CaSiO3–L-methionine configuration, where the interaction occurs through the amino group of the amino acid; the energy value of which was −2058.497 kcal/mol. To confirm the simulation results, the samples were examined using IR spectroscopy. An analysis of the results showed that the interaction of calcium silicate with L-methionine occurs via the formation of a bond through the NH3+ group of the amino acid.
