Low-cost, immediate, general-purpose, and high-throughput (LIGHt) smartphone colorimetric screening assay for water-soluble protein

Yunfei Sha; Yumei Chen; Wenchen Li; Jianhao Zhang; Jiale Wang; Ting Fei; Da Wu; Weiying Lu

Heliyon (Aug 2024)

Low-cost, immediate, general-purpose, and high-throughput (LIGHt) smartphone colorimetric screening assay for water-soluble protein

Yunfei Sha,
Yumei Chen,
Wenchen Li,
Jianhao Zhang,
Jiale Wang,
Ting Fei,
Da Wu,
Weiying Lu

Affiliations

Yunfei Sha: Key Laboratory of Cigarette Smoke, Technology Center of Shanghai Tobacco Group Co. Ltd, Shanghai, 200082, China
Yumei Chen: Institute of Food and Nutraceutical Science, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China
Wenchen Li: Institute of Food and Nutraceutical Science, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China
Jianhao Zhang: Institute of Food and Nutraceutical Science, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China
Jiale Wang: Key Laboratory of Cigarette Smoke, Technology Center of Shanghai Tobacco Group Co. Ltd, Shanghai, 200082, China; Corresponding author.
Ting Fei: Key Laboratory of Cigarette Smoke, Technology Center of Shanghai Tobacco Group Co. Ltd, Shanghai, 200082, China
Da Wu: Key Laboratory of Cigarette Smoke, Technology Center of Shanghai Tobacco Group Co. Ltd, Shanghai, 200082, China
Weiying Lu: Institute of Food and Nutraceutical Science, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China; Corresponding author.

Journal volume & issue: Vol. 10, no. 15
p. e35596

Abstract

Read online

An efficient and rapid method for the detection of total soluble protein in tobacco leaves, utilizing a smartphone-based colorimetric approach has been developed. The proposed low-cost, immediate, general-purpose, and high-throughput (LIGHt) smartphone colorimetric screening assay integrates commercially available microplates, enabling on-site, high-throughput screening of tobacco leaf quality. The study involves preparing protein standard solutions and constructing standard curves using both spectrophotometric and smartphone-based methods. The LIGHt smartphone colorimetry yielded an average relative standard deviation of 10.6 %, a limit of detection of 2 μg/mL, and an average recovery of 93 %. The results demonstrated a comparable performance between intensities from the blue channel and the absorbance values in reflecting protein concentrations, validating the feasibility of utilizing smartphone colorimetry for protein concentration determination. Our approach demonstrates the potential for practical implementation in the field, providing a cost-effective and user-friendly solution for rapid quality assessment in the tobacco industry. The LIGHt smartphone colorimetry enhances quality control practices in the tobacco sector and offers a promising tool for on-site production quality testing in various industries, such as fruits and vegetables.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

About the journal

Abstract

Keywords