Nanomaterial based water degradation is becoming as a promising option in comparison to conventional water degradation methods. MoO _3 nanoparticles have been used as a nano adsorbent for methylene blue (MB) removal from aqueous solution. Here, effect of vanadium (V) element doping in MoO _3 on adsorption activity against MB was studied. 2%, 4%, 6% and 8% of V element doped MoO _3 nanoparticles were synthesized using surfactant free chemical method. All the synthesized nanoparticles were well characterized through different analysis tools to study their structural, morphological, and optical properties. Stability of particles in water with respect to time was also studied by zeta potential. Adsorption activity of all the samples were carried out and 8% doped MoO _3 nanoparticle was found to be most efficient. Moreover, the regeneration and reusability test of 8% doped MoO _3 nanoparticle was also successfully carried out.