Neural Regeneration Research (Jan 2017)

Three-dimensional histology: new visual approaches to morphological changes during neural regeneration

  • Hei Ming Lai,
  • Ho Man Ng,
  • Wutian Wu

DOI
https://doi.org/10.4103/1673-5374.198974
Journal volume & issue
Vol. 12, no. 1
pp. 53 – 55

Abstract

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Three-dimensional (3D) histology utilizes tissue clearing techniques to turn intact tissues transparent, allowing rapid interrogation of tissue architecture in three dimensions. In this article, we summarized the available tissue clearing methods and classified them according to their physicochemical principles of operation, which provided a framework for one to choose the best techniques for various research settings. Recent attempts in addressing various questions regarding the degenerating and regenerating nervous system have been promising with the use of 3D histological techniques.

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