Bio-Protocol (Mar 2017)

3D Stroma Invasion Assay

  • Yvette Coulson-Thomas,
  • Vivien Coulson-Thomas

DOI
https://doi.org/10.21769/BioProtoc.2195
Journal volume & issue
Vol. 7, no. 6

Abstract

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We have developed a 3D co-culture system composed of fibroblasts and colorectal cancer cells that enables us to study the desmoplastic reaction. This method also enables us to study the influence of the desmoplastic reaction on the migration of colorectal cancer cells through the surrounding stroma. This protocol has been previously published (Coulson-Thomas et al., 2011) and is described here in more detail.