Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In Vitro

Svitlana Prylutska; Anna Grebinyk; Stanislav Ponomarenko; Defne Gövem; Vasyl Chumachenko; Nataliya Kutsevol; Mykola Petrovsky; Uwe Ritter; Marcus Frohme; Jacek Piosik; Yuriy Prylutskyy

doi:10.3390/ijms25053069

International Journal of Molecular Sciences (Mar 2024)

Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In Vitro

Svitlana Prylutska,
Anna Grebinyk,
Stanislav Ponomarenko,
Defne Gövem,
Vasyl Chumachenko,
Nataliya Kutsevol,
Mykola Petrovsky,
Uwe Ritter,
Marcus Frohme,
Jacek Piosik,
Yuriy Prylutskyy

Affiliations

Svitlana Prylutska: Department of Plants Physiology, Biochemistry and Bioenergetics, National University of Life and Environmental Sciences of Ukraine, 03041 Kyiv, Ukraine
Anna Grebinyk: Deutsches Elektronen-Synchrotron DESY, Platanenallee 6, 15738 Zeuthen, Germany
Stanislav Ponomarenko: Department of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine
Defne Gövem: Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, 15745 Wildau, Germany
Vasyl Chumachenko: Department of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine
Nataliya Kutsevol: Department of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine
Mykola Petrovsky: Department of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine
Uwe Ritter: Institute of Chemistry and Biotechnology, Technical University of Ilmenau, 98693 Ilmenau, Germany
Marcus Frohme: Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, 15745 Wildau, Germany
Jacek Piosik: Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, 80-307 Gdańsk, Poland
Yuriy Prylutskyy: Department of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine

DOI: https://doi.org/10.3390/ijms25053069
Journal volume & issue: Vol. 25, no. 5
p. 3069

Abstract

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The application of a biocompatible polymer nanocarrier can provide target delivery to tumor tissues, improved pharmacokinetics, controlled drug release, etc. Therefore, the proposed strategy was to use the water-soluble star-like copolymers with a Dextran core and Poly(N-isopropylacrylamide) grafts (D-g-PNIPAM) for conjugation with the widely used chemotherapy drugs in oncology–Cisplatin (Cis-Pt) and Doxorubicin (Dox). The molecular characteristics of the copolymer were received using size-exclusion chromatography. The physicochemical characterization of the D-g-PNIPAM-Cis-Pt (or Dox) nanosystem was conducted using dynamic light scattering and FTIR spectroscopy. Using traditional biochemical methods, a comparative analysis of the enhancement of the cytotoxic effect of free Cis-Pt and Dox in combination with D-g-PNIPAM copolymers was performed in cancer cells of the Lewis lung carcinoma line, which are both sensitive and resistant to Dox; in addition, the mechanism of their action in vitro was evaluated.

Published in International Journal of Molecular Sciences

ISSN: 1661-6596 (Print); 1422-0067 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General); Science: Chemistry
Website: http://www.mdpi.com/journal/ijms

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