Fibrotic Transformation of the Rat Pancreas as Response to the Nitric Oxide Deficiency

Abstract

Non-specific inhibitor of nitric oxide NG-nitro-L-arginine was administrated for the duration of the experiment, and the most significant changes (discirculation and dissecretion) were observed on the 12th day, whereupon the reaction slows due to a compensatory response. On the 30th day of experiment tiny bands of fibrous tissue were formed, which also evidenced by the increase of collagen synthesis markers in blood — protein-bound hydroxyproline (p < 0.05). Incompetence of pancreatic cells was manifested by the reduced activity of pancreatic enzymes (p < 0.05), and the visible hyposecretion of acinar cells on the morphological study. The maximum decrease of nitrite/nitrate concentration was observed after the first day (p < 0.05), with a gradual increase to a maximum on 12th day (p < 0.05).