Molecular inspection of contamination with Salmonella Enteritidis in Tabriz city aviaries

Abstract

Summary The aim of this study was to investigate the occurrence of Salmonella Enteritidis infection between the birds in Tabriz city aviary by molecular method. In this study, the presence of paratyphoid infection in the pet bird market of Tabriz city was investigated for the first time. One hundred six fecal samples were obtained from aviaries in Tabriz urban area. The samples were then transferred to tetrathionate broth culture media. After 24 hours of incubation, samples were transferred to MacConkey agar plates. Samples showing no fermentation of lactose and containing transparent colonies were transferred to salmonella shigella agar plates. In the next stage, the resulting dark colonies, which were determined to be salmonella bacteria, were entered to a multiplex polymerase chain reaction test. Findings showed that 7 (6.6%) out of 106 samples were contaminated with salmonella bacteria. In addition, results of multiplex PCR confirmed Salmonella Enteritidis as the source of Salmonella Enteritidis contamination in 2 (1.88%) samples. This finding shows that a slight degree of contamination with enteritidis infection is present in birds in Tabriz city aviaries. It can be concluded that there is a slight rate of infection by Salmonella Enteritidis in Tabriz city aviaries. Our findings show that this contamination is latent, and necessary measures should be taken to confront it.

Keywords

• tabriz aviary
• bacterial infection
• salmonella enteritidis
• pcr