The Relationship between the Genetic Polymorphisms of Interleukin-33 (IL-33) and Interleukin-1 Receptor-Like 1 (IL1R1) with the Levels of Interleukin-33 in Patients with Asthma and Multiple Sclerosis

Abstract

Background: Recent evidence suggests that the interleukin-33/interleukin-1 receptor-like 1 (IL-33/IL1RL1) axis plays a critical role in autoimmune and inflammatory disorders; however, its mechanistic role in these diseases has not been clearly defined. The present study aimed to investigate the frequency of rs1342326 polymorphism of IL-33 gene and rs10204137 polymorphism of IL1RL1 gene with serum levels of IL-33 in multiple sclerosis (MS), asthma and healthy subjects In Isfahan Province, Iran. Methods: Samples were taken from the patients with asthma (140) or multiple sclerosis (140) and healthy controls (72). Different genotypes of T/G or G/A polymorphisms were studied using the high-resolution melting real-time polymerase chain reaction (HRM real-time PCR) technique. Serum levels of IL-33 were measured via enzyme-linked immunosorbent assay (ELISA). Findings: There was not any relationship between the frequency of polymorphism rs10204137 of IL1RL1 gene and susceptibility to multiple sclerosis or asthma. There was no significant correlation between the frequency of rs1342326 polymorphism of IL-33 gene in healthy subjects compared to those with asthma or multiple sclerosis. Serum levels of IL-33 were higher than in patients with multiple sclerosis or asthma compared to healthy subjects. Conclusion: Serum levels of IL-33 in the two groups of patient increased substantially compared to the controls.

Keywords

• Interleukin-33 (IL-33)
• Multiple sclerosis
• Asthma
• Polymorphism