Genetically stable CRISPR-based kill switches for engineered microbes

Austin G. Rottinghaus; Aura Ferreiro; Skye R. S. Fishbein; Gautam Dantas; Tae Seok Moon

doi:10.1038/s41467-022-28163-5

Nature Communications (Feb 2022)

Genetically stable CRISPR-based kill switches for engineered microbes

Austin G. Rottinghaus,
Aura Ferreiro,
Skye R. S. Fishbein,
Gautam Dantas,
Tae Seok Moon

Affiliations

Austin G. Rottinghaus: Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis
Aura Ferreiro: The Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine
Skye R. S. Fishbein: The Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine
Gautam Dantas: The Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine
Tae Seok Moon: Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis

DOI: https://doi.org/10.1038/s41467-022-28163-5
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 17

Abstract

Read online

Biocontainment is a key to developing safe genetically-engineered microbes (GEMs). Here the authors demonstrate genetically stable CRISPR-based kill switches that control GEMs’ viability in animal hosts, enabling their safe biomedical applications.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal