Identification and expression analysis of UV-B photoreceptor UVR8 genes in Apocynum venetum and A. cannabinum

Jinfeng CHE; Qing ZHANG; Guoqi LI; Boxun XIE; Sheng XIE; Changhai ZHAO; Keyu ZHANG; Xing LIU

doi:10.11931/guihaia.gxzw202208012

Guangxi Zhiwu (Oct 2023)

Identification and expression analysis of UV-B photoreceptor UVR8 genes in Apocynum venetum and A. cannabinum

Jinfeng CHE,
Qing ZHANG,
Guoqi LI,
Boxun XIE,
Sheng XIE,
Changhai ZHAO,
Keyu ZHANG,
Xing LIU

Affiliations

Jinfeng CHE: Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021, China
Qing ZHANG: Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021, China
Guoqi LI: Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021, China
Boxun XIE: Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021, China
Sheng XIE: Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021, China
Changhai ZHAO: Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021, China
Keyu ZHANG: Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021, China
Xing LIU: Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021, China

DOI: https://doi.org/10.11931/guihaia.gxzw202208012
Journal volume & issue: Vol. 43, no. 10
pp. 1876 – 1891

Abstract

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In the processes of plants response to UV-B, the UV-B photoreceptor UVR8 (UV Resistance Locus 8) plays an important role in the regulation of photomorphogenesis, growth and metabolism of plants. To investigate the UV-B photoreceptors of Apocynum plant, this study is conducted to screen and bioinformatically analyze the UV-B photoreceptors UVR8 by the whole genome data of Apocynum venetum and A. cannabinum, and also to analyze the UVR8 gene expression pattern under UV-B stress treatment using transcriptome data. The results were as follows: (1) There were six UVR8 genes in A. venetum, and five UVR8 genes in A. cannabinum. The former was distributed on chromosomes 1, 7, 9 and 11, and the latter on chromosomes 1, 8 and 9. (2) UVR8 proteins were all hydrophilic stable proteins, localized in the nucleus, without transmembrane structure or signal peptides. The secondary structure consists mainly of extended strand, random coil, alpha helix and beta turn. The tertiary structures of AvUVR8b and AcUVR8a were most similar to that of Arabidopsis thaliana and were most closely related to Coffea arabica (CaUVR8) and C. eugenioides (CeUVR8). The gene and protein structures of Apocynum venetum AvUVR8b and A. cannabinum AcUVR8a were highly similar to those of AtUVR8. (3) The expression levels of AvUVR8b and AcUVR8a were up-regulated when the two Apocynum plants were treated with a certain dose of UV-B (17.52 kJ·m-2·d-1). It is speculated that in response to UV-B, the AvUVR8b in A. venetum and the AcUVR8a in A. cannabinum play a major role, respectively. (4) The analysis of cis-acting elements showed that the expression of UVR8 was regulated by light, temperature, moisture, oxygen and hormones. This study will lay a foundation for further research on the gene function of UVR8 in Apocynum, and provide clues to analyze the molecular mechanism of UV-B adaptation in the Apocynum.

Published in Guangxi Zhiwu

ISSN: 1000-3142 (Print)
Publisher: China Science Publishing & Media Ltd. (CSPM)
Country of publisher: China
LCC subjects: Science: Botany
Website: http://www.guihaia-journal.com/gxzwen/ch/index.aspx

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