Nature Communications (Feb 2019)

Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo

  • Jes Dreier,
  • Marco Castello,
  • Giovanna Coceano,
  • Rodrigo Cáceres,
  • Julie Plastino,
  • Giuseppe Vicidomini,
  • Ilaria Testa

DOI
https://doi.org/10.1038/s41467-019-08442-4
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 11

Abstract

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Long acquisition time and high illumination intensities needed in super-resolution imaging often generate photobleaching and phototoxicity. Here the authors develop a non-deterministic scanning approach based on a real-time feedback system that enables faster acquisition with lower light doses for in vivo imaging.