Isolation and characterization of a MAPKK gene from Penaeus monodon in response to bacterial infection and low-salinity challenge

Hongdi Fan; Yundong Li; Qibin Yang; Song Jiang; Lishi Yang; Jianhua Huang; Shigui Jiang; Falin Zhou

Aquaculture Reports (Jul 2021)

Isolation and characterization of a MAPKK gene from Penaeus monodon in response to bacterial infection and low-salinity challenge

Hongdi Fan,
Yundong Li,
Qibin Yang,
Song Jiang,
Lishi Yang,
Jianhua Huang,
Shigui Jiang,
Falin Zhou

Affiliations

Hongdi Fan: Tropical Fishery Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, China
Yundong Li: Tropical Fishery Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, China
Qibin Yang: Tropical Fishery Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China
Song Jiang: Tropical Fishery Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, China
Lishi Yang: Tropical Fishery Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, China
Jianhua Huang: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, China
Shigui Jiang: Tropical Fishery Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, China
Falin Zhou: Tropical Fishery Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, China; Corresponding author at: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingang West Road, Guangzhou, 510300, China.

Journal volume & issue: Vol. 20
p. 100671

Abstract

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Mitogen-activated protein kinase kinase (MAPKK), a component of the MAPK signal transduction pathway, plays a crucial role in stress resistance and immunity. Herein, a MAPKK cDNA from Penaeus monodon (black tiger shrimp) was cloned (PmMAPKK), and its effect on bacterial infection and low-salinity stress was investigated. The full-length PmMAPKK cDNA was 3665 bp long, with a 1224-bp open reading frame (ORF), encoding a 408-amino acid (aa) protein. Results revealed that PmMAPKK was expressed in all examined tissues of P. monodon, including hepatopancreas, gills, and lymphoid organ. The PmMAPKK expression level was found to be highest in ovary (approximately 8.7-fold higher than in hepatopancreas), and lowest in testis (approximately 0.54 times that in hepatopancreas). In early developmental stages of P. monodon, PmMAPKK expression was highest during the zygote stage. From the zoea1 stage onward, a steady decrease in expression was registered. Meanwhile, in the bacterial infection experiment, the hepatopancreas was found to be more sensitive than gills after bacterial infection. Following infection with Staphylococcus aureus and Vibrio anguillarum, PmMAPKK expression was appeared significantly higher than that in the control group (P < 0.05). However, infection with Vibrio harveyi caused a significant decrease in the expression level compared to the control group (P < 0.05). Furthermore, low-salinity environment significantly altered PmMAPKK expression in hepatopancreas and gills (P < 0.05). Silencing of PmMAPKK expression caused the mortality of P. monodon to increase under low-salinity stress conditions. Furthermore, the ERK, JNK, and p38 signaling pathways were activated to varying degrees concomitantly, reflecting the complex regulatory mechanisms in the MAPK signaling pathway. Hence, the results of the present study indicate that PmMAPKK is involved in the innate immune mechanism of P. monodon as well as its adaptation to low-salinity environments.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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