Effect of buffalo PDC-109 on caudal epididymal spermatozoa of bubaline species

Abstract

A study was conducted to assess the effect of buffalo seminal PDC-109 on its cauda epididymal spermatozoa. PDC-109 protein was purified by applying two-step chromatography procedures and included into epididymal spermatozoa. Epididymal ejaculates were splited into four groups as Gr 1: Control (without PDC), Gr 2: 20 μg/mL PDC-109, Gr 3: 40 μg/mL PDC-109 and Gr 4: 80 μg/mL PDC-109. Semen quality parameters (SQPs) and in vitro fertility assay were evaluated. Significant improvement in post-thaw SQPs was observed in Gr 2 than in Gr 3, however, Gr 1 had significantly higher value than other groups. It is concluded that PDC-109 has dose dependent effect as increased dose causes detrimental effect in buffalo cauda epididymal sperm.

Keywords

• Buffalo
• Caudal epididymal spermatozoa
• In vitro capacitation
• In vitro fertilization
• PDC-109