Frontiers in Pediatrics (May 2021)

Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia

  • Chien-Chou Hsiao,
  • Chien-Chou Hsiao,
  • Chien-Chou Hsiao,
  • Cheng-Han Lee,
  • Rei-Cheng Yang,
  • Rei-Cheng Yang,
  • Jia-Yuh Chen,
  • Tzu-Cheng Su,
  • Yu-Jun Chang,
  • Ching-Yuang Lin,
  • Yi-Giien Tsai,
  • Yi-Giien Tsai,
  • Yi-Giien Tsai

DOI
https://doi.org/10.3389/fped.2021.616452
Journal volume & issue
Vol. 9

Abstract

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Background: Heat shock protein-70 (Hsp-70) exhibits cytoprotective effects against oxidative stress-induced airway injury. This study aimed to examine Hsp-70 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) from tracheal aspirates (TA) in very low-birth weight (VLBW) preterm infants to predict the development of bronchopulmonary dysplasia (BPD).Methods: This birth cohort study enrolled 109 VLBW preterm infants, including 32 infants who developed BPD. Hsp-70 and 8-OHdG concentrations from TA were measured by immunoassay. The apoptosis of TA epithelial cells obtained on Day 28 after birth was measured using annexin-V staining assay.Results: Hsp-70 and 8-OHdG levels in TA fluid were persistently increased from Day 1 to Day 28 of life in the BPD group. Multiple linear regression analysis demonstrated that BPD was significantly associated with gestational age, respiratory distress syndrome, and TA Hsp-70 and 8-OHdG levels on post-natal Day 28. The TA Hsp-70 level positively correlated with TA 8-OHdG level on the Day 1 (r = 0.47) and Day 28 of life (r = 0.68). Incubation of recombinant Hsp-70 with primary epithelial cells derived from TA of patients decreased hydrogen peroxide-induced epithelial cell death.Conclusions: Hsp-70 levels are associated with a state of oxidative injury in the development of BPD.

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