Investigation of Protective Properties of the Constructed Recombinant Biplasmid Strain of <i>Vibrio cholerae</i> O139 Serogroup Producing Cholera Toxin B Subunit and <i>Escherichia coli</i> Colonization Factor CFA/1

Abstract

The constructed avirulent biplasmid Vibrio cholerae strain KM182 producing cholera toxin B subunit and Escherichia coli colonization factor CFA/1 (providing for antitoxic and anti-colonization immunity formation, correspondently) was demonstrated to protect immunized model laboratory animals from experimental cholera caused by the virulent Vibrio cholerae O139 strain. The optimal doses for immunization and challenging were determined.

Keywords

• <i>vibrio cholerae</i> o139 serogroup
• plasmid
• ritard method
• cholera toxin b subunit
• colonization factor cfa/1