Engineering threshold-based selection systems

Katherine H Pedone; Vanessa González-Pérez; Luciana E Leopold; Neal R Rasmussen; Channing J Der; Adrienne D Cox; Shawn Ahmed; David J Reiner

doi:10.1093/g3journal/jkab234

G3: Genes, Genomes, Genetics (Jul 2021)

Engineering threshold-based selection systems

Katherine H Pedone,
Vanessa González-Pérez,
Luciana E Leopold,
Neal R Rasmussen,
Channing J Der,
Adrienne D Cox,
Shawn Ahmed,
David J Reiner

Affiliations

Katherine H Pedone: Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
Vanessa González-Pérez: Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA
Luciana E Leopold: Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA
Neal R Rasmussen: Institute of Biosciences and Technology, College of Medicine, Texas A&M Health Science Center, Houston, TX 77030, USA
Channing J Der: Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
Adrienne D Cox: Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
Shawn Ahmed: ORCiD; Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA
David J Reiner: ORCiD; Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA

DOI: https://doi.org/10.1093/g3journal/jkab234
Journal volume & issue: Vol. 11, no. 9

Abstract

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AbstractUsing model organisms to identify novel therapeutic targets is frequently constrained by pre-existing genetic toolkits. To expedite positive selection for identification of novel downstream effectors, we engineered conditional expression of activated CED-10/Rac to disrupt Caenorhabditis elegans

Published in G3: Genes, Genomes, Genetics

ISSN: 2160-1836 (Online)
Publisher: Oxford University Press
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General): Genetics
Website: https://academic.oup.com/g3journal

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