Multiplexed analysis of EV reveals specific biomarker composition with diagnostic impact

Joshua D. Spitzberg; Scott Ferguson; Katherine S. Yang; Hannah M. Peterson; Jonathan C. T. Carlson; Ralph Weissleder

doi:10.1038/s41467-023-36932-z

Nature Communications (Mar 2023)

Multiplexed analysis of EV reveals specific biomarker composition with diagnostic impact

Joshua D. Spitzberg,
Scott Ferguson,
Katherine S. Yang,
Hannah M. Peterson,
Jonathan C. T. Carlson,
Ralph Weissleder

Affiliations

Joshua D. Spitzberg: Center for Systems Biology, Massachusetts General Hospital
Scott Ferguson: Center for Systems Biology, Massachusetts General Hospital
Katherine S. Yang: Center for Systems Biology, Massachusetts General Hospital
Hannah M. Peterson: Center for Systems Biology, Massachusetts General Hospital
Jonathan C. T. Carlson: Center for Systems Biology, Massachusetts General Hospital
Ralph Weissleder: Center for Systems Biology, Massachusetts General Hospital

DOI: https://doi.org/10.1038/s41467-023-36932-z
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 12

Abstract

Read online

Multiplexed analyses of near single EVs is currently challenging. Here the authors report the method MASEV, multiplexed analysis of EVs, to interrogate thousands of individual EVs during 5 cycles of multi-channel fluorescence staining for 15 EV biomarkers.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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