Einstein (São Paulo) ()

Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria

  • Rodolfo Patussi Correia,
  • Laiz Cameirão Bento,
  • Ana Carolina Apelle Bortolucci,
  • Anderson Marega Alexandre,
  • Andressa da Costa Vaz,
  • Daniela Schimidell,
  • Eduardo de Carvalho Pedro,
  • Fabricio Simões Perin,
  • Sonia Tsukasa Nozawa,
  • Cláudio Ernesto Albers Mendes,
  • Rodrigo de Souza Barroso,
  • Nydia Strachman Bacal

DOI
https://doi.org/10.1590/S1679-45082016AO3641
Journal volume & issue
Vol. 14, no. 3
pp. 366 – 373

Abstract

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ABSTRACT Objective: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. Methods: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. Results: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. Conclusion: High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones.

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