Journal of Advanced Veterinary and Animal Research (Sep 2021)

First case report on molecular detection of Trypanosoma lewisi in an urban rat in Kelantan, Malaysia: An accidental finding

  • Intan Noor Aina Kamaruzaman,
  • Hong Wei Ting,
  • Muhammad Aiman Mohd Mokhtar,
  • Yong Kai Yuan,
  • Azim Wafiy Gulam Shah,
  • Fathin Faahimaah Abdul Hamid,
  • Che Wan Salma Che Wan Zalati,
  • Nurshahirah Shaharulnizim,
  • Mohd Farhan Hanif Reduan,
  • Luqman Abu-Bakar

DOI
https://doi.org/10.5455/javar.2021.h531
Journal volume & issue
Vol. 8, no. 3
pp. 435 – 439

Abstract

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Objective: This case report highlights the first detection of Trypanosoma lewisi, a blood protozoan parasite found in an urban rat in Kota Bharu, Kelantan. Materials and Methods: Rat trapping was carried out within the Kota Bharu vicinity near a local wet market. A total of 38 rats were captured and subjected to peripheral blood smearing using Giemsa stain. Positive rats were sent for histopathological analysis for the evaluation of the organ samples. Results: The presence of trypanosomes was found in one sample from a blood smear. This was connected to a histological lesion on kidney tissues, which revealed a high concentration of trypanosomes. Additionally, the positive sample was confirmed as T. lewisi based on molecular diagnosis via polymerase chain reaction and subsequent sequencing and phylogenetic analysis. Conclusions: This finding serves as a baseline for further surveillance on T. lewisi population among urban rats in Kelantan and possible zoonotic transmission to humans. [J Adv Vet Anim Res 2021; 8(3.000): 435-439]

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