An in vitro platform for engineering and harnessing modular polyketide synthases

Takeshi Miyazawa; Melissa Hirsch; Zhicheng Zhang; Adrian T. Keatinge-Clay

doi:10.1038/s41467-019-13811-0

Nature Communications (Jan 2020)

An in vitro platform for engineering and harnessing modular polyketide synthases

Takeshi Miyazawa,
Melissa Hirsch,
Zhicheng Zhang,
Adrian T. Keatinge-Clay

Affiliations

Takeshi Miyazawa: Department of Molecular Biosciences, The University of Texas at Austin
Melissa Hirsch: Department of Chemistry, The University of Texas at Austin
Zhicheng Zhang: Department of Chemistry, The University of Texas at Austin
Adrian T. Keatinge-Clay: Department of Molecular Biosciences, The University of Texas at Austin

DOI: https://doi.org/10.1038/s41467-019-13811-0
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 7

Abstract

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A robust platform to study modular polyketide synthases (PKSs) in vitro is still unavailable. Here, the authors report the reconstitution of the venemycin PKS, engineer hybrid venemycin/pikromycin PKSs, and obtain much improved yields through employing the updated module boundaries.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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