Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair
Motoki Okamoto,
Sayako Matsumoto,
Ayato Sugiyama,
Kei Kanie,
Masakatsu Watanabe,
Hailing Huang,
Manahil Ali,
Yuki Ito,
Jiro Miura,
Yujiro Hirose,
Koichiro Uto,
Mitsuhiro Ebara,
Ryuji Kato,
Aika Yamawaki-Ogata,
Yuji Narita,
Shigetada Kawabata,
Yusuke Takahashi,
Mikako Hayashi
Affiliations
Motoki Okamoto
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan
Sayako Matsumoto
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan
Ayato Sugiyama
Department of Basic Medicinal Science, Nagoya University, Chikusa, Nagoya 464-8601, Japan
Kei Kanie
Department of Basic Medicinal Science, Nagoya University, Chikusa, Nagoya 464-8601, Japan
Masakatsu Watanabe
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan
Hailing Huang
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan
Manahil Ali
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan
Yuki Ito
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan
Jiro Miura
Division for Interdisciplinary Dentistry, Osaka University Dental Hospital, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan
Yujiro Hirose
Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan
Koichiro Uto
International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1 Chome-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
Mitsuhiro Ebara
International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1 Chome-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
Ryuji Kato
Department of Basic Medicinal Science, Nagoya University, Chikusa, Nagoya 464-8601, Japan
Aika Yamawaki-Ogata
Department of Cardiac Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan
Yuji Narita
Department of Cardiac Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan
Shigetada Kawabata
Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan
Yusuke Takahashi
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan
Mikako Hayashi
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan
Vital pulp therapy is an important endodontic treatment. Strategies using growth factors and biological molecules are effective in developing pulp capping materials based on wound healing by the dentin-pulp complex. Our group developed biodegradable viscoelastic polymer materials for tissue-engineered medical devices. The polymer contents help overcome the poor fracture toughness of hydroxyapatite (HAp)-facilitated osteogenic differentiation of pulp cells. However, the composition of this novel polymer remained unclear. This study evaluated a novel polymer composite, P(CL-co-DLLA) and HAp, as a direct pulp capping carrier for biological molecules. The biocompatibility of the novel polymer composite was evaluated by determining the cytotoxicity and proliferation of human dental stem cells in vitro. The novel polymer composite with BMP-2, which reportedly induced tertiary dentin, was tested as a direct pulp capping material in a rat model. Cytotoxicity and proliferation assays revealed that the biocompatibility of the novel polymer composite was similar to that of the control. The novel polymer composite with BMP-2-induced tertiary dentin, similar to hydraulic calcium-silicate cement, in the direct pulp capping model. The BMP-2 composite upregulated wound healing-related gene expression compared to the novel polymer composite alone. Therefore, we suggest that novel polymer composites could be effective carriers for pulp capping.