Study of gastrointestinal neoplasms, histopathologic and immunohistochemical correlation using HER-2/NEU, CK-7 and CK-20

Abstract

Background & Aims: This study aimed to determine the significance of immunohistochemistry (IHC) and its correlation using HER-2/NEU and cytokeratins in a histopathologic study of GI neoplasms. Materials & Methods: This retrospective- prospective study was conducted on 50 gastric biopsies along with patient details and stained with H and E method for histopathology. Immunohistochemistry with HER-2/NEU, cytokeratin-7 and cytokeratin-20 were also done. Results: 60% were males among total sample size. Adenocarcinoma prevalence was 76%, followed by mucinous adenocarcinoma in 10%, signet cell ring cell carcinoma in 6%, and mixed adeno-neuroendocrine tumor in 4%. Sarcoma and papillary adenocarcinoma contributed to 2% each. Among study population, 60% were of grade II, 32% were of grade III, and 6% were of grade I. Low-grade sarcoma was present in 2%. Well differentiated tumors were 6%, moderately differentiated tumors were 60%, and poorly differentiated tumors were 32%. Among study population, 16% were positive for Her 2 neu, 20% were positive for CK7, and CK20 was positive in 28%, respectively. Among the study population, 2.63% of adenocarcinomas, all of mixed adeno-neuroendocrine, 60% of mucinous adenocarcinomas, and 66.66% of signet ring cell carcinomas were positive for Her2neu. 18.42% of adenocarcinomas, 50% of mixed adeno-neuroendocrine and 66.66% of signet ring cell carcinomas were positive for CK7. 21.05% of adenocarcinomas, all of mucinous adenocarcinomas, and 33.33% of signet ring cell carcinomas were positive for CK20. Conclusion: 16% were positive for Her 2 neu, 20% were positive for CK7, and CK20 was positive among 28%. There was no correlation between the histopathology status and receptor positivity.

Keywords

• ck7
• ck20
• gastric adenocarcinoma
• her2/neu
• immunohistochemistry