Application of real-time PCR to characterize the viral load, genotypes, resistance of hepatitis B virus at Dong Thap hospital

Abstract

Hepatitis B is caused by the hepatitis B virus (HBV) and may be either acute or chronic. Chronic hepatitis B virus (HBV) infection is the major global cause of chronic hepatitis, as a result, which leads to cirrhosis and increases the risk of liver cancer development (hepatocellular carcinoma). Recent advances in the diagnosis and treatment of hepatitis B have enormously contributed to reduce the side effects of this disease in which the real-time PCR technique had the significant contribution todiagnosis, monitoring and therapy. In the current study, therefore, our purpose is the application of real-time PCR method to detect hepatitis B viral load, genotypes as well as lamivudine (LAM) and adefovir (ADV) resistance in patients at Dong Thap Hospital. The results figured that the mean age of HBV patients was 37.0 ± 0.34, the proportion of male infected was 59.0%. In addition, our results also showed that the rate of the patients with the normal ALT level and HBeAg negative were 51.2% and 23.0%, respectively. Moreover, the patients who were not indicated for treatment were approximately 30.0%. Among the group of HBsAg positive, the rate of HBV-DNA positive was 82.0% and a majority rate of 59.1% was HBV genotype B. The group of high viral load in those samples was equal to or greater than 20,000 IU/ml (36.0%). The total rate resistant mutation occurred in 56.1%, the rate of LAM resistant mutations was the most value of 86.0% and ADV resistant mutations were 68.0%. LAM resistant mutation 204I occurred at 84.0%. ADV resistant mutation A181T was the highest rate of 68.0%. Resistance mutations often associated with a higher proportion of HBeAg positive and the high viral loads. Meanwhile, the influence of genotype infections on the clinical, para-clinical characteristics were not clear.

Keywords

• đột biến kháng thuốc
• kiểu gen
• tải lượng virus
• real-time pcr
• virus viêm gan b