The Association between rs763763348 and rs190628533 SNPs in CLCA4 Gene and Azoospermia

Abstract

Background and Objective: Spermatogenesis is a complex phenomenon that is influenced by various genes. One of these genes that is likely to be effective in causing azoospermia is CLCA4. This study was conducted to investigate the association between rs763763348 and rs190628533 single nucleotide polymorphisms in CLCA4 gene and azoospermia in Iranian men. Methods: In this cross-sectional study, blood samples were collected from 100 men suffering from non‐obstructive azoospermia referred to Jihad Daneshgahi Infertility Treatment Center in Qom, as well as 100 fertile men who had at least one child and had healthy sperm test. The DNA of the samples was extracted by salting out method. Then, Tetra-primer ARMS PCR technique was used to check single nucleotide polymorphisms and the relationship between single nucleotide polymorphisms (SNPs) and male infertility was investigated. Findings: The mean age of healthy people was 33.12±2.789 years and the mean age of patients was 32.54±2.571 years. The mean level of FSH hormone in healthy and sick subjects was 6.86±1.214 and 15.05±2.078, respectively (p<0.0001). The mean level of LH hormone in healthy people (4.12±1.04) and in sick people (11.44±1.54) showed a significant relationship (p<0.0001). In all studied subjects, the genotype of 100% of the subjects regarding rs190628533 was CC and the genotype of 100% of subjects regarding rs763334876 was GG. There was no significant difference between the healthy and sick groups in the evaluation of these two SNPs. Conclusion: According to the results of this study, single nucleotide polymorphisms of rs190628533 and rs763334876 are not the cause of infertility due to azoospermia among Iranian men.

Keywords

• azoospermia
• single nucleotide polymorphism
• clca4 gene
• male infertility.