Initial genetic characterisation of rye (secale cereale L.) in the Gene Bank of the Republic of Srpska

Abstract

The Gene Bank of the Republic of Srpska (Genetic Resources Institute of the University of Banja Luka) was established in 2009. The characterization of accessions with molecular markers began during 2010. This paper presents the initial results of genetic characterization of five rye accessions. The analysis was performed using RAPD markers (Randomly Amplified Polymorphic DNA). 13 amplified loci were obtained by polymerase chain reaction and 7 were polymorphic which represents 54% of total polymorphism. The coefficient of genetic similarity (according to Jaccard) ranged from 0.68 for the Nanid variety, 0.73 for the Selge variety and 0.78%, for the Albedo variety. The coefficient of similarity between accessions 1 and 2 (unknown name) was 1, which means that these two accessions have identical allelic profiles for the analyzed loci. The Albedo variety and duplicate accessions are the most genetically similar whilst the Nanid variety was the least genetically similar to other accessions under study.

Keywords

• rapd markers
• duplicated accessions
• genetic similarity