Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment

Jianxiong Chan; Gita  V. Soraya; Lauren Craig; Shah  M. Uddin; Marian Todaro; Duc  H. Huynh; Chathurika  D. Abeyrathne; Lyudmila Kostenko; James McCluskey; Efstratios Skafidas; Patrick Kwan

doi:10.3390/s19163543

Sensors (Aug 2019)

Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment

Jianxiong Chan,
Gita V. Soraya,
Lauren Craig,
Shah M. Uddin,
Marian Todaro,
Duc H. Huynh,
Chathurika D. Abeyrathne,
Lyudmila Kostenko,
James McCluskey,
Efstratios Skafidas,
Patrick Kwan

Affiliations

Jianxiong Chan: Department of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, Australia
Gita V. Soraya: Department of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, Australia
Lauren Craig: Department of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, Australia
Shah M. Uddin: Department of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, Australia
Marian Todaro: Department of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, Australia
Duc H. Huynh: Department of Electrical and Electronic Engineering, Melbourne School of Engineering, The University of Melbourne, Victoria 3010, Australia
Chathurika D. Abeyrathne: Department of Electrical and Electronic Engineering, Melbourne School of Engineering, The University of Melbourne, Victoria 3010, Australia
Lyudmila Kostenko: Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Victoria 3010, Australia
James McCluskey: Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Victoria 3010, Australia
Efstratios Skafidas: Department of Electrical and Electronic Engineering, Melbourne School of Engineering, The University of Melbourne, Victoria 3010, Australia
Patrick Kwan: Department of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, Australia

DOI: https://doi.org/10.3390/s19163543
Journal volume & issue: Vol. 19, no. 16
p. 3543

Abstract

Read online

Pre-treatment screening of individuals for human leukocyte antigens (HLA) HLA-B*57:01 is recommended for the prevention of life-threatening hypersensitivity reactions to abacavir, a drug widely prescribed for HIV treatment. However, the implementation of screening in clinical practice is hindered by the slow turnaround time and high cost of conventional HLA genotyping methods. We have developed a biosensor platform using interdigitated electrode (IDE) functionalized with a monoclonal antibody to detect cells expressing HLA-B*57:01. This platform was evaluated using cell lines and peripheral blood mononuclear cells expressing different HLA-B alleles. The functionalized IDE sensor was able to specifically capture HLA-B*57:01 cells, resulting in a significant change in the impedance magnitude in 20 min. This IDE platform has the potential to be further developed to enable point-of-care HLA-B*57:01 screening.

Published in Sensors

ISSN: 1424-8220 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: http://www.mdpi.com/journal/sensors

About the journal

Abstract

Keywords