Effect of bacterial culture filtrates with antifungical <em>in vitro</em> activity in the <em>Musa</em> spp.- <em>Mycosphaerella fijiensis</em> interaction

Abstract

Although certain bacterial species may be in vitro antagonistic of Mycosphaerella fijiensis, as it was demonstrated, they are discrete results that have their effect on the Musa spp.-M. fijiensis interaction. The results will help to design new strategies for genetic improvement and development of biological products for control of black Sigatoka. The aim of this study was to determine the effect of bacterial culture filtrates, with antifungal activity in vitro against M. fijiensis in the interaction between the pathogen and plants of Musa spp., artificially inoculated in greenhouse. Culture filtrates of two strains of Bacillus sp. (CCIBP-B.1 and CCIBP-M27) with in vitro antifungal activity against M. fijiensis were used, with two treatments: application of bacterial culture filtrates three hours before and three days after inoculation of M. fijiensis. The application of bacterial culture filtrates delayed the development of the disease. It also depended on the strain and time of application. Keywords: artificial inoculation, Bacillus sp., biocontrol, Black Sigatoka