Complete Genome Sequences of <i>Klebsiella michiganensis</i> and <i>Citrobacter farmeri</i>, KPC-2-Producers Serially Isolated from a Single Patient

Abstract

Carbapenemase-producing Enterobacterales, including KPC-2 producers, have become a major clinical problem. During an outbreak in Quebec City, Canada, KPC-2-producing Klebsiella michiganensis and Citrobacter farmeri were isolated from a patient six weeks apart. We determined their complete genome sequences. Both isolates carried nearly identical IncN2 plasmids with blaKPC-2 on a Tn4401b element. Both strains also carried IncP1 plasmids, but that of C. farmeri did not carry a Beta-lactamase gene, whereas that of K. michiganensis carried a second copy of blaKPC-2 on Tn4401b. These results suggest recent plasmid transfer between the two species and a recent transposition event.

Keywords

• <i>Klebsiella michiganensis</i>
• <i>Citrobacter farmeri</i>
• KPC-2
• carbapenemase
• plasmid
• transposon