High-affinity chromodomains engineered for improved detection of histone methylation and enhanced CRISPR-based gene repression

G. Veggiani; R. Villaseñor; G. D. Martyn; J. Q. Tang; W. M. Krone; J. Gu; C. Chen; M. L. Waters; K. H. Pearce; T. Baubec; S. S. Sidhu

doi:10.1038/s41467-022-34269-7

Nature Communications (Nov 2022)

High-affinity chromodomains engineered for improved detection of histone methylation and enhanced CRISPR-based gene repression

G. Veggiani,
R. Villaseñor,
G. D. Martyn,
J. Q. Tang,
W. M. Krone,
J. Gu,
C. Chen,
M. L. Waters,
K. H. Pearce,
T. Baubec,
S. S. Sidhu

Affiliations

G. Veggiani: The Anvil Institute
R. Villaseñor: Division of Molecular Biology, Biomedical Center Munich, Ludwig-Maximilians-University
G. D. Martyn: The Anvil Institute
J. Q. Tang: The Anvil Institute
W. M. Krone: Department of Chemistry, University of North Carolina at Chapel Hill, CB 3290
J. Gu: The Anvil Institute
C. Chen: The Anvil Institute
M. L. Waters: Department of Chemistry, University of North Carolina at Chapel Hill, CB 3290
K. H. Pearce: Center for Integrative Chemical Biology and Drug Discovery, Division of Chemical Biology and Medicinal Chemistry, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill
T. Baubec: Department of Molecular Mechanisms of Disease, University of Zurich
S. S. Sidhu: The Anvil Institute

DOI: https://doi.org/10.1038/s41467-022-34269-7
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

Read online

Engineered chromodomains are promising probes to analyze histone methylation. Here the authors designed high-affinity chromodomains for enhanced genome-wide binding analysis, live-cell imaging and ultra-potent CRISPR-based gene repression.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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