Investigation on Biological Characteristics and Aging Phenotype of SHJHhr Mice

Abstract

Objective To measure and analyze biological characteristics and aging phenotype of SHJHhr mice and provide basic data for the application of the mouse model in aging mechanisms research and antiaging drug development.MethodsWith ICR mice of the same age as control group, the body mass growth data of SHJHhr mice at the age of 3 to 16 weeks, the reproduction ability of 1 to 4 fetuses and the life cycle of SHJHhr mice were measured. Blood routine (30 items) and serum biochemical indexes (25 items) of 6-week-old SHJHhr mice were measured. The venous blood of 8-week-old SHJHhr mice was collected for flow cytometry analysis to determine the content of immune cells. The aging bone structure of the cancellous bone and bone mineral density of SHJHhr mice aged 4, 8 and 26 weeks were measured by micro-CT. Histopathological changes of bone and joint of 8-week-old mice were observed.ResultsCompared with ICR mice, the female and male body mass of SHJHhr mice were significantly lower at the age of 16 weeks (P < 0.05), and the reproductive performance of female mice was low (P < 0.01) or did not have normal reproductive capacity. The shortest survival time of SHJHhr mice was 57 weeks and the longest was 71 weeks, which was shorter than those of normal ICR mice, showing obvious rapid aging phenomenon. At the same time, some physiological and biochemical indexes of blood and pathological changes of bone and cartilage tissues also showed the accelerated aging and abnormality of animal physiological functions.ConclusionSHJHhr mice have some biological characteristics of rapid aging as well as some physiological and pathological changes caused by aging.

Keywords

• spontaneous rapid aging
• bio-characteristics
• aging phenotype
• shjhhr mice