Translational Medicine of Aging (Jan 2019)
Rb analog Whi5 regulates G1 to S transition and cell size but not replicative lifespan in budding yeast
- Matthew M. Crane,
- Mitsuhiro Tsuchiya,
- Ben W. Blue,
- Jared D. Almazan,
- Kenneth L. Chen,
- Siobhan R. Duffy,
- Alexandra Golubeva,
- Annaiz M. Grimm,
- Alison M. Guard,
- Shauna A. Hill,
- Ellen Huynh,
- Ryan M. Kelly,
- Michael Kiflezghi,
- Hyunsung D. Kim,
- Mitchell Lee,
- Ting-I. Lee,
- Jiayi Li,
- Bao M.G. Nguyen,
- Riley M. Whalen,
- Feng Y. Yeh,
- Mark McCormick,
- Brian K. Kennedy,
- Joe R. Delaney,
- Matt Kaeberlein
Affiliations
- Matthew M. Crane
- Department of Pathology, University of Washington, Seattle, WA, USA
- Mitsuhiro Tsuchiya
- Department of Pathology, University of Washington, Seattle, WA, USA
- Ben W. Blue
- Department of Pathology, University of Washington, Seattle, WA, USA
- Jared D. Almazan
- Department of Pathology, University of Washington, Seattle, WA, USA
- Kenneth L. Chen
- Department of Pathology, University of Washington, Seattle, WA, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA; Medical Scientist Training Program, University of Washington, Seattle, WA, USA
- Siobhan R. Duffy
- Department of Pathology, University of Washington, Seattle, WA, USA
- Alexandra Golubeva
- Department of Pathology, University of Washington, Seattle, WA, USA
- Annaiz M. Grimm
- Department of Pathology, University of Washington, Seattle, WA, USA
- Alison M. Guard
- Department of Pathology, University of Washington, Seattle, WA, USA
- Shauna A. Hill
- Department of Pathology, University of Washington, Seattle, WA, USA
- Ellen Huynh
- Department of Pathology, University of Washington, Seattle, WA, USA
- Ryan M. Kelly
- Department of Pathology, University of Washington, Seattle, WA, USA
- Michael Kiflezghi
- Department of Pathology, University of Washington, Seattle, WA, USA
- Hyunsung D. Kim
- Department of Pathology, University of Washington, Seattle, WA, USA
- Mitchell Lee
- Department of Pathology, University of Washington, Seattle, WA, USA
- Ting-I. Lee
- Department of Pathology, University of Washington, Seattle, WA, USA
- Jiayi Li
- Department of Pathology, University of Washington, Seattle, WA, USA
- Bao M.G. Nguyen
- Department of Pathology, University of Washington, Seattle, WA, USA
- Riley M. Whalen
- Department of Pathology, University of Washington, Seattle, WA, USA
- Feng Y. Yeh
- Department of Pathology, University of Washington, Seattle, WA, USA
- Mark McCormick
- Department of Biochemistry and Molecular Biology, University of New Mexico, Albuquerque, NM, USA
- Brian K. Kennedy
- Buck Institute for Research on Aging, Novato, CA, USA
- Joe R. Delaney
- Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA
- Matt Kaeberlein
- Department of Pathology, University of Washington, Seattle, WA, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA; Corresponding author. Department of Pathology, University of Washington, Seattle, WA, USA.
- Journal volume & issue
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Vol. 3
pp. 104 – 108
Abstract
An increase in cell size with age is a characteristic feature of replicative aging in budding yeast. Deletion of the gene encoding Whi5 results in shortened duration of G1 and reduced cell size, and has been previously suggested to increase replicative lifespan. Upon careful analysis of multiple independently derived haploid and homozygous diploid whi5Δ mutants, we find no effect on lifespan, but we do confirm the reduction in cell size. We suggest that instead of antagonizing lifespan, the elongated G1 phase of the cell cycle during aging may actually play an important role in allowing aged cells time to repair accumulating DNA damage. Keywords: Cell cycle regulation, Replicative aging, Saccharomyces cerevisiae
