Plant Stress (Mar 2024)
Combined application of biochar and metal-tolerant bacteria alleviates cadmium toxicity by modulating the antioxidant defense mechanism and physicochemical attributes in rice (Oryza sativa L.) grown in cadmium-contaminated soil
Abstract
Agricultural soil contamination by heavy metal (cadmium) is a widespread issue and causes deleterious effects on crops. One of the most effective and ecofriendly methods for cadmium detoxification is microbe-assisted phytoremediation and soil amendment with biochar. The current study investigated the combined effect of biochar (Parthenium hysterophorus L.) and microbes (Bacillus cereus strain B2) in alleviating cadmium toxicity in rice grown in cadmium-contaminated soil (0, 10, 15, and 20 mg/kg). Results revealed that cadmium caused damage to plant health. Maximum reduction in plant biomass, gas exchange parameters, and chlorophyll content was observed on cadmium 20 mg/kg. As compared with the sole application of biochar (2 %) and microbes, the greatest plant height (33.68%), photosynthetic rate (23.52 %), stomatal conductance (48.93 %), water use efficiency (21.41 %), transpiration rate (58.87 %), chlorophyll a (74.81 %), chlorophyll b (31.28 %), total chlorophyll (60.53 %), and carotenoids (26.97 %) were recorded with combined application of biochar and microbes. Similarly, superoxidase (SOD), catalase (CAT), peroxidase (POD), and absorbate peroxidase (APX) activity was significantly boosted by combined application of microbes and biochar (2 %), which were 33.90 %, 24.18 %, 51.70 %, and 19.06 %, respectively, while significant reduction was found in the ROS (16.60 %), (20.06 %) and MDA (26.08 %) content in rice plants. However, cadmium concentrations in different parts of plant were decreased at higher concentrations of biochar (2 %) with microbes. These findings indicate that combined application of Bacillus cereus strain B2 and biochar could be a promising strategy for reducing rice crop cadmium toxicity and boosting its growth, physiology, and defense system efficiently as compared with sole application.
