European Journal of Medical Research (Sep 2009)

Detection of promoter hypermethylation of the CpG island of E-cadherin in gastric cardiac adenocarcinoma

  • Guo W,
  • Dong Z,
  • Guo Y,
  • Kuang G,
  • Yang Z,
  • Chen Z

DOI
https://doi.org/10.1186/2047-783X-14-10-453
Journal volume & issue
Vol. 14, no. 10
p. 453

Abstract

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Abstract Aim Abnormal hypermethylation of CpG islands associated with tumor suppressor genes can lead to transcriptional silencing in neoplasia. The aim of this study was to investigate the promoter methylation and expression of E-cadherin gene in gastric cardiac adenocarcinoma (GCA). Methods A nested MSP approach, immunohistochemistry method and RT-PCR were used respectively to examine the methylation status of the 5' CpG island of E-cadherin, its protein expression and mRNA expression in tumors and corresponding normal tissues. Results E-cadherin was methylated in 63 of 92 (68.5%) tumor specimens, which was significantly higher than that in corresponding normal tissues (P Conclusions High methylation status of the 5' CpG island of E-cadherin gene may be one of the mechanisms in the development of gastric cardiac adenocarcinoma.

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