Endometriosis Associated-miRNome Analysis of Blood Samples: A Prospective Study
Sofiane Bendifallah,
Yohann Dabi,
Stéphane Suisse,
Léa Delbos,
Mathieu Poilblanc,
Philippe Descamps,
Francois Golfier,
Ludmila Jornea,
Delphine Bouteiller,
Cyril Touboul,
Anne Puchar,
Emile Daraï
Affiliations
Sofiane Bendifallah
Clinical Research Group (GRC) Paris 6: Centre Expert Endométriose (C3E), Department of Obstetrics and Reproductive Medicine, Hôpital Tenon, Sorbonne University (GRC6 C3E SU), 4 rue de la Chine, 75020 Paris, France
Yohann Dabi
Clinical Research Group (GRC) Paris 6: Centre Expert Endométriose (C3E), Department of Obstetrics and Reproductive Medicine, Hôpital Tenon, Sorbonne University (GRC6 C3E SU), 4 rue de la Chine, 75020 Paris, France
Stéphane Suisse
Ziwig, 19 rue Reboud, 69003 Lyon, France
Léa Delbos
Endometriosis Expert Center—Pays de la Loire, Department of Obstetrics and Reproductive Medicine—CHU d’Angers, 49100 Angers, France
Mathieu Poilblanc
Endometriosis Expert Center—Steering Committee of the EndAURA Network, Department of Obstetrics and Reproductive Medicine, Lyon South University Hospital, Lyon Civil Hospices, 69310 Pierre Bénite, France
Philippe Descamps
Endometriosis Expert Center—Pays de la Loire, Department of Obstetrics and Reproductive Medicine—CHU d’Angers, 49100 Angers, France
Francois Golfier
Endometriosis Expert Center—Steering Committee of the EndAURA Network, Department of Obstetrics and Reproductive Medicine, Lyon South University Hospital, Lyon Civil Hospices, 69310 Pierre Bénite, France
Ludmila Jornea
Paris Brain Institute—Institut du Cerveau—ICM, Inserm U1127, CNRS UMR 7225, AP-HP—Hôpital Pitié-Salpêtrière, Sorbonne University, 75006 Paris, France
Delphine Bouteiller
Genotyping and Sequencing Core Facility, iGenSeq, Institut du Cerveau et de la Moelle épinière, ICM, Hôpital Pitié-Salpêtrière, 47-83 Boulevard de l’Hôpital, 75013 Paris, France
Cyril Touboul
Clinical Research Group (GRC) Paris 6: Centre Expert Endométriose (C3E), Department of Obstetrics and Reproductive Medicine, Hôpital Tenon, Sorbonne University (GRC6 C3E SU), 4 rue de la Chine, 75020 Paris, France
Anne Puchar
Department of Obstetrics and Reproductive Medicine, Hôpital Tenon, Sorbonne University, 4 rue de la Chine, 75020 Paris, France
Emile Daraï
Clinical Research Group (GRC) Paris 6: Centre Expert Endométriose (C3E), Department of Obstetrics and Reproductive Medicine, Hôpital Tenon, Sorbonne University (GRC6 C3E SU), 4 rue de la Chine, 75020 Paris, France
The aim of our study was to describe the bioinformatics approach to analyze miRNome with Next Generation Sequencing (NGS) of 200 plasma samples from patients with and without endometriosis. Patients were prospectively included in the ENDO-miRNA study that selected patients with pelvic pain suggestive of endometriosis. miRNA sequencing was performed using an Novaseq6000 sequencer (Illumina, San Diego, CA, USA). Small RNA-seq of 200 plasma samples yielded ~4228 M raw sequencing reads. A total of 2633 miRNAs were found differentially expressed. Among them, 8.6% (n = 229) were up- or downregulated. For these 229 miRNAs, the F1-score, sensitivity, specificity, and AUC ranged from 0–88.2%, 0–99.4%, 4.3–100%, and 41.5–68%, respectively. Utilizing the combined bioinformatic and NGS approach, a specific and broad panel of miRNAs was detected as being potentially suitable for building a blood signature of endometriosis.
