Antibodies targeting the European lobster (Palinurus elephas) vitellogenin developed by mRNA isolation and in-silico-designed antigenic peptides
Faustina B. Cannea,
Maria Cristina Follesa,
Cristina Porcu,
Rossano Rossino,
Alessandra Olianas,
Antonio Rescigno,
Alessandra Padiglia
Affiliations
Faustina B. Cannea
Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Biomedical section, University of Cagliari, Cittadella Universitaria di Monserrato, 09042 Monserrato, Cagliari, Italy
Maria Cristina Follesa
Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Marine Bioecology section, University of Cagliari, via T. Fiorelli 1, 09126 Cagliari, Italy
Cristina Porcu
Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Marine Bioecology section, University of Cagliari, via T. Fiorelli 1, 09126 Cagliari, Italy
Rossano Rossino
Dipartimento di Scienze Mediche e Sanità Pubblica (DSMSP), University of Cagliari, AOU Presidio microcitemico via Jenner, 09121 Cagliari, Italy
Alessandra Olianas
Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Biomedical section, University of Cagliari, Cittadella Universitaria di Monserrato, 09042 Monserrato, Cagliari, Italy
Antonio Rescigno
Dipartimento di Scienze Biomediche (DiSB), Cittadella Universitaria di Monserrato, 09042 Monserrato, Cagliari, Italy
Alessandra Padiglia
Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Biomedical section, University of Cagliari, Cittadella Universitaria di Monserrato, 09042 Monserrato, Cagliari, Italy
Vitellogenin is an essential protein involved in ovary maturation in many animals. Detection of this protein correlated with reproductive capacity may be important if carried out on marine organisms such as the red spiny lobster Palinurus elephas, a crustacean that is an economically important crop from wild fish catches. Moreover, in recent years, vitellogenin has assumed an important role as a possible biomarker of marine environmental pollution, as its expression levels can be influenced by the presence of similar estrogen pollutants and can affect the reproductive sphere of marine organisms such as crustaceans. The P. elephas vitellogenin protein and its coding gene have never been isolated, so there is little information about its presence in this lobster. The aim of the present study was to develop a molecular strategy to create, for the first time, an antibody for the detection and quantization of vitellogenin in P. elephas.
