Prevalence of Leishmania infection in refugee camps: A serological and molecular study in Gambella and Benishangul-Gumuz, Ethiopia.

Abstract

BackgroundLeishmaniasis, transmitted by sandflies, causes a severe health threat in East African refugee camps. High mobility, poor conditions and limited healthcare access heighten the risk of visceral leishmaniasis (VL) among refugees. Though, data on infection prevalence is remains limited. This study aims to determine the prevalence of Leishmania infection in refugee camps in Benishangul Gumuz and Gambella regions to improve VL detection and guide effective control strategies in humanitarian settings.MethodsA cross-sectional study was conducted from May to August 2023 in four refugee camps to determine Leishmania infection using DAT and rtPCR on blood samples. Sociodemographic and clinical data were collected using structured questionnaires. Ethical approval was granted, and informed consent was obtained. Data were analyzed using SPSS v23, with associations assessed using logistic regression and Chi-square tests at a 0.05 significance level. Continuous variables summarized by median and interquartile range (IQR).ResultThe study included 1,223 participants (440 from Tsore camp and 220 from Sherkole in Benishangul Gumuz Region; 288 from Kule camp and 275 from Terkidi in Gambella Region), most of whom were from South Sudan (66.7%) and the majority were females (56.5%). 17.8% of the participants reported fever, with no spleen or liver enlargement and 0.2% lymph node swelling. Real-time PCR positivity was significantly higher in Tsore (14.6%, χ² = 21.4, p ConclusionLeishmania infection was prevalent in refugee camps in Gambella and Benishangul Gumuz regions. Asymptomatic cases and low parasite loads were common, highlighting the need for active case detection, intervention including treatment and vector control to manage VL transmission effectively.